Facscalibur manual pdf

18 BD FACSCalibur Instructions for Use Optics Components and Theory of Operation The standard BD FACSCalibur system includes a mW, nm, argon-ion laser and detectors for three fluorescent parameters. The FL4 option provides a second nm, red-diode laser and an additional detector. Laser light is focused onto the flow cell by a focusing. FACSCalibur, and FACSVantage-SE SOP January 6, 6 As mentioned previously, aspects of this project are conducted in a manual fashion in the laboratories listed, and are therefore authorized by the individual PI‟s BUAs. Administrative Controls Training: Training of all personnel using the FACS will be documented and covered. The manual assumes the reader has a basic understanding of flow cytometric data acquisition xP3+ 3 Color FACScan or FACSCalibur with Pulse Processing xP5 2-laser, 5 Color FACScan or FACSCalibur with Pulse Processing • DigitalCytometers Supported DxP6 2-laser, 6 Color FACScan or FACSCalibur with Digital Electronics.

The BD FACSCalibur System: The Gold Standard That Continues to Evolve The BD FACSCalibur™ flow cytometer is another BD Biosciences first: a multicolor benchtop flow cytometry system that is capable of both analyzing and sorting. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the BD FACSCalibur. FACSCalibur System User’s Guide v Preface FACSCalibur™ is the Becton Dickinson Immunocytometry Systems (BDIS) modular benchtop flow cytometer designed for applications ranging from routine clinical to advanced research. This modular system features advanced capabilities, such as the Sorting and FL4 options in an easy-to-use system. The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single bench-top system. Designed with multicolor application support in mind, the BD FACSCalibur flow cytometer provides flexibility to support a wide.

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